Euglena gracilis is a commercially successful microalgal species, as it is a rich source of valuable products such as amino acids, paramylon, carotenoids, ascorbic acid, α-tocopherol and polyunsaturated fatty acids. E. gracilis is regards as a novel and renewable feedstock for biofuel and biodiesel production have been aroused due to its high lipid biosynthesis efficiency, appropriate fatty acid composition, fast lipid extraction process and positive environment impact. An environment-friendly and cost-effective approach to improving lipid productivity without sacrificing microalgal growth was established. Syringic acid (SA) and p-coumaric acid (p-CA) are two major lignin hydrolysates, widely distributed in the wastewater from downstream industries of lignin. In this study, their potential in regulating the growth and valuable metabolites biosynthesis of microalga Euglena gracilis was evaluated. The cell growth was increased by 1.63 and 1.93 times at the optimal dosage of 0.5 g⋅L-1 SA and 0.3 g⋅L-1p-CA, respectively. Cell morphology also varied under the two treatments, reflecting the changes in cellular physiological status. Moreover, increased chlorophyll a content was only observed under p-CA treatment, indicating the influences of these compounds on photosynthesis were different. Fourier-transform infrared spectroscopy analysis revealed the fluctuations in the macromolecular pools. The variation trend of carbohydrate content was different under the treatment of the p-CA and SA, demonstrating that different phenolic compounds will determine different biosynthetic pathways and the flow of carbon to metabolites in microalgal cells. However, lipid biosynthesis was improved by both of the phenolic acid's treatments, which would be favorable to the biofuel production since the cell biomass was not sacrificed for lipid accumulation.

Figure 1: Utilizing of lignin in promoting growth and lipid production of Euglena gracilis

Jiangyu Zhu, Xiaomiao Tan, Halimatun Saadiah Hafid, Minato Wakisaka (2023). A novel strategy to promote microalgal growth and lipid productivity by supplementation of lignin related phenolic elicitors. Fuel 334 (2): 126775

Artikel penuh: https://doi.org/

Tarikh Input: 29/12/2023 | Kemaskini: 29/12/2023 | ainzubaidah